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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/07/2019 |
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Actualizado : |
10/07/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BENÍTEZ-GALEANO, M.J.; VALLET, T.; CARRAU, L.; HERNÁNDEZ-RODRÍGUEZ, L.; BERTALMIO, A.; RIVAS, F.; RUBIO, L.; MAESO, D.; VIGNUZZI, M.; MORATORIO, G.; COLINA, R. |
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Afiliación : |
MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratory of Molecular Virology, University of the Republic, Salto, Uruguay.; THOMAS VALLET, Institut Pasteur, Viral Populations and Pathogenesis, Centre National de la Recherche Scientifique, Paris, France; LUCÍA CARRAU, Institut Pasteur, Viral Populations and Pathogenesis, Centre National de la Recherche Scientifique, Paris, France; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO VIGNUZZI, Institut Pasteur, Viral Populations and Pathogenesis, Centre National de la Recherche Scientifique, Paris, France; GONZALO MORATORIO, Institut Pasteur, Viral Populations and Pathogenesis, Centre National de la Recherche Scientifique, Paris, France; RODNEY COLINA, Laboratory of Molecular Virology, University of the Republic, Salto, Uruguay. |
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Título : |
Complete genome sequence of a novel recombinant Citrus tristeza virus, a resistance-breaking isolate from Uruguay. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Genome Announcements, 1 May 2018, Volume 6, Issue 22, Article number e00442-18. OPEN ACCESS. |
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ISSN : |
2169-8287 |
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DOI : |
10.1128/genomeA.00442-18 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 April 2018 / Accepted 24 April / 2018 Published 31 May 2018.
Accession number(s). The genomic sequence for isolate CTV DSST-17 was deposited in GenBank under accession number MH186146. |
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Contenido : |
ABSTRACT.
We report here the complete genome sequence of a Citrus tristeza virus (CTV) from Uruguay, sequenced by using Illumina and Sanger sequencing technology. This CTV DSST-17 genome clustered within genotype resistance breaking (RB) and presents two recombination events.
© 2018 Benítez-Galeano et al. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13013/1/Genome-Announcements.-2018.-10.1128genomeA.00442-18.pdf
https://mra.asm.org/content/6/22/e00442-18
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Marc : |
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024 7 $a10.1128/genomeA.00442-18$2DOI
100 1 $aBENÍTEZ-GALEANO, M.J.
245 $aComplete genome sequence of a novel recombinant Citrus tristeza virus, a resistance-breaking isolate from Uruguay.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 19 April 2018 / Accepted 24 April / 2018 Published 31 May 2018. Accession number(s). The genomic sequence for isolate CTV DSST-17 was deposited in GenBank under accession number MH186146.
520 $aABSTRACT. We report here the complete genome sequence of a Citrus tristeza virus (CTV) from Uruguay, sequenced by using Illumina and Sanger sequencing technology. This CTV DSST-17 genome clustered within genotype resistance breaking (RB) and presents two recombination events. © 2018 Benítez-Galeano et al.
650 $aCITRUS
700 1 $aVALLET, T.
700 1 $aCARRAU, L.
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
700 1 $aBERTALMIO, A.
700 1 $aRIVAS, F.
700 1 $aRUBIO, L.
700 1 $aMAESO, D.
700 1 $aVIGNUZZI, M.
700 1 $aMORATORIO, G.
700 1 $aCOLINA, R.
773 $tGenome Announcements, 1 May 2018, Volume 6, Issue 22, Article number e00442-18. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 4 |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500
001 1051279
005 2019-10-15
008 2014 bl uuuu u00u1 u #d
022 $a1948-5948
024 7 $a10.4172/1948-5948.1000155$2DOI
100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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